Multiple Choice
During which stage of transcription do the following processes take place?
1. RNA polymerase binds to the promoter.
2. The RNA transcript is released.
3. The RNA transcript extended.
3. Energy & Cell Processes
Steps of Transcription
Back3. Energy & Cell Processes
Steps of Transcription
- Textbook QuestionIn a particular bacterial species, temperature-sensitive conditional mutations cause expression of a wild-type phenotype at one growth temperature and a mutant phenotype at another—typically higher—temperature. Imagine that when a bacterial cell carrying such a mutation is shifted from low to high growth temperatures, RNA polymerases in the process of elongation complete transcription normally, but no new transcripts can be started. The mutation in this strain most likely affects:a. the terminator sequenceb. the start codonc. sigmad. one of the polypeptides of the core RNA polymerase1views
- Textbook QuestionIn a particular bacterial species, temperature-sensitive conditional mutations cause expression of a wild-type phenotype at one growth temperature and a mutant phenotype at another—typically higher—temperature. Imagine that when a bacterial cell carrying such a mutation is shifted from low to high growth temperatures, RNA polymerases in the process of elongation complete transcription normally, but no new transcripts can be started. The mutation in this strain most likely affects:a. the terminator sequenceb. the start codonc. sigmad. one of the polypeptides of the core RNA polymerase1views
- Multiple Choice
Which of the following properties is NOT shared by RNA polymerase and DNA polymerase?
6views - Textbook QuestionThe nucleotide shown here is called cordycepin triphosphate. It is a natural product of a fungus that is used in traditional medicines. If cordycepin triphosphate is added to a cell-free transcription reaction, the nucleotide is added onto the growing RNA chain but then no more nucleotides can be added. Examine the structure of cordycepin and explain why it ends transcription.4views
- Textbook QuestionIf you wanted to use αα-amanitin to shut down 95 percent of transcription by RNA polymerase II, roughly what concentration of αα-amanitin would you use? Note that the scale on the x-axis of the graph in Question 13 is logarithmic rather than linear, so that each tick mark shows a tenfold higher concentration.
- Multiple ChoiceOne strand of a DNA molecule has the following sequence: 3′-AGTACAAACTATCCACCGTC-5′. In order for that strand to be transcribed, there would have to be a specific recognition sequence, called a(n) __________, to the left of the DNA sequence indicated.2views
- Textbook QuestionEating even a single death cap mushroom (Amanita phalloides) can be fatal due to a compound called αα-amanitin, a toxin that inhibits transcription.What would you predict to be the immediate outcome of adding αα-amanitin to a cell?a. reduced DNA synthesisb. reduced production of one or more types of RNAc. reduced binding of tRNAs to anticodonsd. reduced rate of translocation of ribosomes translating mRNA
- Multiple Choice
What happens during the initiation step of DNA transcription?
3views - Textbook Question
Each of the following statements about protein synthesis is false. Correct each to make a true statement.
The enzyme RNA polymerase builds a strand of transfer RNA, whose codons are complementary to DNA's triplets.
- Textbook QuestionIn eukaryotic cells, transcription cannot begin untila. the two DNA strands have completely separated and exposed the promoter.b. several transcription factors have bound to the promoter.c. the 5′ caps are removed from the mRNA.d. the DNA introns are removed from the template.
- Textbook Questionα-Amanitin inhibits transcription by binding inside an RNA polymerase to a region other than the active site that catalyzes addition of a nucleotide to the RNA chain. Based on the model of RNA polymerase shown in Figure 17.3, predict how the toxin might function to inhibit transcription.3views
- Multiple Choice
Transcription in eukaryotes requires which of the following molecules in addition to RNA polymerase?
3views - Textbook QuestionThe RNA polymerase enzyme binds to , initiating transcription.a. amino acids;b. tRNA;c. the promoter sequence;d. the ribosome1views
- Multiple Choice
During transcription of DNA to RNA:
4views