Skip to main content
Ch. 9 - Biotechnology & DNA Technology
Tortora - Microbiology: An Introduction 14th Edition
Tortora14th EditionMicrobiology: An IntroductionISBN: 9780138200398Not the one you use?Change textbook
All textbooksTortora 14th EditionCh. 9 - Biotechnology & DNA TechnologyProblem 8
Chapter 9, Problem 8

A population of cells carrying a desired plasmid.
a. Antisense
b. Clone
c. Library
d. Southern blot
e. Vector

Verified step by step guidance
1
Understand the key terms provided: 'antisense', 'clone', 'library', 'Southern blot', and 'vector'. Each term has a specific meaning in molecular biology and microbiology.
Focus on the phrase 'A population of cells carrying a desired plasmid.' This implies multiple cells that all contain the same plasmid, which is a circular DNA molecule used in cloning.
Recall that a 'clone' in microbiology refers to a group of genetically identical cells derived from a single ancestor cell, often carrying the same plasmid.
Match the statement to the term that best fits the description of identical cells carrying the same plasmid, which is 'clone'.
Confirm that other options do not fit: 'antisense' relates to RNA sequences, 'library' is a collection of DNA fragments, 'Southern blot' is a DNA detection method, and 'vector' is the DNA molecule used to carry foreign DNA but not the population of cells itself.

Verified video answer for a similar problem:

This video solution was recommended by our tutors as helpful for the problem above.
Video duration:
1m
Was this helpful?

Key Concepts

Here are the essential concepts you must grasp in order to answer the question correctly.

Clone

A clone refers to a population of genetically identical cells derived from a single ancestor cell, often carrying the same plasmid or DNA sequence. In molecular biology, cloning is used to amplify a specific DNA fragment within host cells.
Recommended video:
Guided course
02:25
Introduction to DNA Cloning

Plasmid

A plasmid is a small, circular DNA molecule independent of chromosomal DNA, commonly found in bacteria. It can replicate autonomously and is often used as a vector to carry foreign genes into host cells for cloning or expression.
Recommended video:
Guided course
05:57
F Plasmids

Vector

A vector is a DNA molecule, such as a plasmid or virus, used to transfer genetic material into a host cell. Vectors contain necessary elements for replication and selection, enabling the propagation and expression of inserted genes.
Recommended video:
Guided course
03:41
Cloning with Recombinant DNA
Related Practice
Textbook Question

Self-replicating DNA for transmitting a gene from one organism to another.

a. Antisense

b. Clone

c. Library

d. Southern blot

e. Vector

1
views
Textbook Question

Pieces of human DNA stored in yeast cells.

a. Antisense

b. Clone

c. Library

d. Southern blot

e. Vector

Textbook Question

Describe an rDNA experiment in two or three sentences. Use the following terms: intron, exon, DNA, mRNA, cDNA, RNA polymerase, reverse transcriptase.

1
views
Textbook Question

You are attempting to insert a gene for saltwater tolerance into a plant by using the Ti plasmid. In addition to the desired gene, you add a gene for tetracycline resistance (tet) to the plasmid. What is the purpose of the tet gene?

4
views
Textbook Question

List at least two examples of the use of rDNA in medicine and in agriculture.

6
views
Textbook Question

How does RNAi “silence” a gene?

1
views