Match the following people to their scientific/medical contribution:
Note: Choices may be used more than once or not at all.

Indicate the true statements and then correct the false statements so that they are true.

a. The Gram stain is a simple stain.

b. Simple stains reveal information about size, shape, and arrangement.

c. Bright field microscopy requires a stained sample.

d. Dark field microscopy requires a stained sample.

e. The acid-fast stain detects peptidoglycan in the cell walls of certain bacteria.

f. Gram-positive bacteria have a thin peptidoglycan layer in the cell wall.

Indicate which form of electron microscopy is being described: SEM, TEM, or both.

a. Images the external surface of a specimen

b. Sample must be thinly sliced prior to viewing

c. Provides details about internal structures of the sample

d. Can be used to image viruses

e. Provides black-and-white images

f. Specimen is dead

Assume you are asked to view and draw all samples at a final magnification of 1000x. If the ocular lens is 10x, what objective lens should be in place as you draft your drawings?

Select the most accurate statement.

a. The Gram stain is a simple stain.

b. Bacterial endospores appear green from the acid-fast stain.

c. The acid-fast stain is used to detect bacteria that have a capsule.

d. Bacterial flagella can only be seen with electron microscopes.

e. The mordant in the Gram stain is iodine.