Textbook Question
The CRISPR-associated enzyme Cas9 __________ .
a. cuts RNA at a specific nucleotide sequence
b. cuts DNA at a specific nucleotide sequence
c. is likely derived from a bacteriophage
d. All of the above are correct.
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Ch. 8 - Recombinant DNA Technology
Bauman6th EditionMicrobiology with Diseases by TaxonomyISBN: 9780134832302
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Table of contents
- Ch. 1 - A Brief History of Microbiology31
- Ch. 2 - The Chemistry of Microbiology28
- Ch. 3 - Cell Structure and Function39
- Ch. 4 - Microscopy, Staining, and Classification25
- Ch. 5 - Microbial Metabolism51
- Ch. 6 - Microbial Nutrition and Growth37
- Ch. 7 - Microbial Genetics51
- Ch. 8 - Recombinant DNA Technology21
- Ch. 9 - Controlling Microbial Growth in the Environment36
- Ch. 10 - Controlling Microbial Growth in the Body: Antimicrobial Drugs21
- Ch. 11 - Characterizing and Classifying Prokaryotes29
- Ch. 12 - Characterizing and Classifying Eukaryotes34
- Ch. 13 - Characterizing and Classifying Viruses, Viroids, and Prions23
- Ch. 14 - Infection, Infectious Diseases, and Epidemiology35
- Ch. 15 - Innate Immunity34
- Ch. 16 - Adaptive Immunity21
- Ch. 17 - Immunization and Immune Testing29
- Ch. 18 - Immune Disorders22
- Ch. 19 - Pathogenic Gram-Positive Bacteria25
- Ch. 20 - Pathogenic Gram-Negative Cocci and Bacilli28
- Ch. 21 - Rickettsias, Chlamydias, Spirochetes, and Vibrios25
- Ch. 22 - Pathogenic Fungi47
- Ch. 23 - Parasitic Protozoa, Helminths, and Arthropod Vectors44
- Ch. 24 - Pathogenic DNA Viruses23
- Ch. 25 - Pathogenic RNA Viruses30
- Ch. 26 - Applied and Industrial Microbiology29
- Ch. 27 - Microbial Ecology and Microbiomes22
Bauman6th EditionMicrobiology with Diseases by TaxonomyISBN: 9780134832302Not the one you use?Change textbook
Chapter 8, Problem 5
Which recombinant DNA technique is used to replicate copies of a DNA molecule?
a. PCR
b. Gel electrophoresis
c. Electroporation
d. Reverse transcription
Verified step by step guidance1
Understand the purpose of each technique listed: PCR, Gel electrophoresis, Electroporation, and Reverse transcription.
Recall that PCR (Polymerase Chain Reaction) is a method used to amplify or make many copies of a specific DNA segment.
Recognize that Gel electrophoresis is used to separate DNA fragments based on size, not to replicate DNA.
Know that Electroporation is a technique to introduce DNA into cells by applying an electric field, not for DNA replication.
Understand that Reverse transcription is the process of converting RNA into complementary DNA (cDNA), not for replicating DNA molecules.
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Key Concepts
Here are the essential concepts you must grasp in order to answer the question correctly.
Polymerase Chain Reaction (PCR)
PCR is a technique used to amplify specific DNA sequences, creating millions of copies from a small initial sample. It involves repeated cycles of denaturation, annealing of primers, and extension by DNA polymerase, enabling rapid and precise DNA replication in vitro.
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01:31
Introduction to Polymerase Chain Reaction
Gel Electrophoresis
Gel electrophoresis is a method for separating DNA fragments based on size by applying an electric field to a gel matrix. It is used to analyze DNA but does not replicate or amplify DNA molecules.
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07:58Gel Electrophoresis
Electroporation
Electroporation is a technique that uses electrical pulses to create temporary pores in cell membranes, allowing DNA or other molecules to enter cells. It facilitates DNA uptake but does not itself replicate DNA.
Related Practice
Textbook Question
Which of the following techniques is used regularly in the study of genomics?
a. Clones are selected using a vector with two genetic markers.
b. Genes are inserted to produce an antigenic protein from a pathogen.
c. Fluorescent nucleotide bases are sequenced.
d. Defective organs are replaced with those made in animal hosts.
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Textbook Question
List three potential problems of recombinant DNA technology.
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Textbook Question
Which of the following would be most useful in following gene expression in a yeast cell?
a. Southern blot
b. PCR
c. DNA microarray
d. Restriction enzymes
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Textbook Question
Describe a CRISPR system and its usefulness.
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Textbook Question
Indicate which of the following are true and which are false. Rewrite any false statements to make them true by changing the underlined words.
__________ Gel electrophoresis is used in DNA microarrays.
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