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Ch. 8 - Recombinant DNA Technology
Bauman - Microbiology with Diseases by Taxonomy 6th Edition
Bauman6th EditionMicrobiology with Diseases by TaxonomyISBN: 9780134832302Not the one you use?Change textbook
All textbooksBauman 6th EditionCh. 8 - Recombinant DNA TechnologyProblem 2
Chapter 8, Problem 2

Indicate which of the following are true and which are false. Rewrite any false statements to make them true by changing the underlined words.
__________ Restriction enzymes act at specific nucleotide sequences within a double-stranded DNA molecule.

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1
Identify the key concept in the statement: restriction enzymes and their action on DNA.
Recall that restriction enzymes, also known as restriction endonucleases, recognize specific nucleotide sequences in double-stranded DNA and cut at or near these sites.
Evaluate the statement: 'Restriction enzymes act at specific nucleotide sequences within a double-stranded DNA molecule.' This is a true statement because restriction enzymes do target specific sequences in double-stranded DNA.
Since the statement is true, no rewriting is necessary.
Summarize: The statement is true because restriction enzymes recognize and cleave specific sequences in double-stranded DNA, which is fundamental to their role in molecular biology.

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Key Concepts

Here are the essential concepts you must grasp in order to answer the question correctly.

Restriction Enzymes

Restriction enzymes, also known as restriction endonucleases, are proteins that recognize specific short DNA sequences and cut the DNA at or near these sites. They are essential tools in molecular biology for DNA cloning and analysis.
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Recognition Sites in DNA

Restriction enzymes target specific nucleotide sequences called recognition sites, which are usually palindromic and found within double-stranded DNA. These sites determine where the enzyme will cleave the DNA molecule.
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Double-Stranded DNA Structure

DNA is composed of two complementary strands forming a double helix. Restriction enzymes require this double-stranded structure to identify and cut at their specific recognition sequences accurately.
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Related Practice
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Indicate which of the following are true and which are false. Rewrite any false statements to make them true by changing the underlined words.

__________ A thermocycler separates molecules based on their size, shape, and electrical charge.

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Textbook Question

Describe three ways scientists use synthetic nucleic acids.

Textbook Question

Indicate which of the following are true and which are false. Rewrite any false statements to make them true by changing the underlined words.

__________ Restriction enzymes inhibit the movement of DNA at specific sites.

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Textbook Question

After scientists exposed cultures of Penicillium to agents X, Y, and Z, they examined the type and amount of penicillin produced by the altered fungi to find the one that is most effective. Agents X, Y, and Z were probably __________ .


a. recombinant cells

b. competent

c. mutagens

d. phages

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Textbook Question

A DNA gene synthesized from an RNA template is ___________ .


a. reverse transcriptase

b. complementary DNA

c. recombinant DNA

d. probe DNA

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Why is cloning a practical technique for medical researchers?

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