Multiple Choice
Why is a DNA polymerase from a thermophilic bacterium used in PCR?
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The Steps of PCR
Multiple Choice
Which of the following correctly lists the steps in order for one cycle of the polymerase chain reaction (PCR)?
A
Denature DNA; Add DNA polymerase; Anneal primers; Add dNTPs; Extend primers.
B
Anneal primers; Denature DNA; Extend primers.
C
Extend primers; Anneal primers; Denature DNA.
D
Denature DNA; Anneal primers; Extend primers utilizing a thermostable DNA polymerase.
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