Ch.17 Nucleic Acids and Protein Synthesis

Timberlake - Chemistry: An Introduction to General, Organic, and Biological Chemistry 14th Edition

Timberlake14th EditionChemistry: An Introduction to General, Organic, and Biological ChemistryISBN: 9781292472249Not the one you use?Change textbook

Timberlake 14th Edition

Ch.17 Nucleic Acids and Protein Synthesis

Problem 67b

Chapter 17, Problem 67b

In sickle-cell anemia, a point mutation in the mRNA for hemoglobin results in the replacement of glutamate with valine in the resulting hemoglobin molecule. Why does the replacement of one amino acid cause such a drastic change in biological function?

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Understand the role of amino acids in protein structure: Amino acids are the building blocks of proteins, and their sequence determines the protein's structure and function. Even a single change in the amino acid sequence can alter the protein's properties.

Identify the chemical properties of glutamate and valine: Glutamate is a polar, negatively charged amino acid, while valine is a nonpolar, hydrophobic amino acid. This difference in chemical properties is key to understanding the impact of the mutation.

Explain the effect of the mutation on hemoglobin's structure: The replacement of glutamate with valine introduces a hydrophobic region on the surface of the hemoglobin molecule. This change disrupts the normal interactions between hemoglobin molecules.

Describe the resulting aggregation of hemoglobin molecules: The hydrophobic valine causes hemoglobin molecules to stick together, forming long, rigid fibers. These fibers distort the shape of red blood cells, giving them the characteristic sickle shape.

Connect the structural change to biological function: The sickle-shaped red blood cells are less flexible and can block blood flow in small vessels, leading to the symptoms of sickle-cell anemia, such as pain, organ damage, and reduced oxygen transport.

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Key Concepts

Here are the essential concepts you must grasp in order to answer the question correctly.

Point Mutation

A point mutation is a change in a single nucleotide base pair in DNA or RNA. In the case of sickle-cell anemia, this mutation alters the codon for glutamate to one that codes for valine. Such mutations can have significant effects on protein structure and function, as they may lead to the incorporation of different amino acids, which can change the protein's properties.

Protein Structure and Function

Proteins are composed of chains of amino acids that fold into specific three-dimensional shapes, which are crucial for their function. The sequence of amino acids determines how the protein folds and its overall stability. In sickle-cell anemia, the substitution of glutamate (a hydrophilic amino acid) with valine (a hydrophobic amino acid) alters the hemoglobin's structure, leading to the formation of rigid fibers that distort red blood cells.

Biological Impact of Hemoglobin Changes

Hemoglobin is responsible for transporting oxygen in the blood. Changes in its structure due to mutations can impair its ability to bind and release oxygen effectively. In sickle-cell anemia, the altered hemoglobin (HbS) leads to sickling of red blood cells, causing blockages in blood vessels, reduced oxygen delivery, and various health complications, illustrating how a single amino acid change can have profound biological consequences.

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Textbook Question

How is a gene for a particular protein inserted into a plasmid?

Textbook Question

Consider the following segment of mRNA produced by the normal order of DNA nucleotides:

ACA UCA CGG GUA

e. What is the amino acid order if an insertion mutation adds a G to the beginning of the mRNA segment?

Textbook Question

A point mutation in the mRNA for an enzyme results in the replacement of leucine with alanine in the resulting enzyme molecule. Why does this change in amino acids have little effect on the biological activity of the enzyme?

Textbook Question

A point mutation changes a codon in the mRNA for an enzyme from GCC to GCA. Why is there no change in the amino acid order in the protein?

Textbook Question

Consider the following segment of mRNA produced by the normal order of DNA nucleotides:

ACA UCA CGG GUA

f. What is the amino acid order if a deletion mutation removes the A at the beginning of the mRNA segment?

Textbook Question

A specific virus contains RNA as its genetic material.

a. Why might reverse transcription be used in the life cycle of this type of virus?