Multiple Choice
What is the primary purpose of gel electrophoresis in molecular biology?
18. Biotechnology
Gel Electrophoresis
Multiple Choice
Gel electrophoresis separates fragments of DNA based on which characteristic?
A
Sequence.
B
Level of methylation.
C
Size.
D
All of the above.
Verified step by step guidance1
Understand the principle of gel electrophoresis: It is a technique used to separate DNA fragments based on their size. DNA molecules are negatively charged due to their phosphate backbone, and when an electric field is applied, they migrate towards the positive electrode.
Consider the structure of the gel: The gel is typically made of agarose or polyacrylamide, which acts as a sieve. Smaller DNA fragments move faster and travel further through the gel matrix compared to larger fragments.
Recognize the role of size in separation: The primary characteristic that affects the movement of DNA fragments in gel electrophoresis is their size. Larger fragments encounter more resistance and move slower, while smaller fragments move more easily through the gel.
Sequence and methylation level are not directly involved in separation: While the sequence and methylation level can affect the properties of DNA, they do not influence the separation process in gel electrophoresis. The separation is purely based on size.
Conclude with the correct answer: Based on the principles of gel electrophoresis, the correct characteristic for separation is size, as it determines how far each fragment will travel in the gel.
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